Lysophosphatidic acid improves development of porcine somatic cell nuclear transfer embryos

This study was conducted to investigate whether lysophosphatidic acid (LPA) could improve the development of porcine somatic cell nuclear transfer (SCNT) embryos. Porcine SCNT-derived embryos were cultured in chemically defined polyvinyl alcohol (PVA)-based zygote medium (PZM)-4 without or with LPA, and development, proliferation potential, apoptosis, expression levels pluripotent markers evaluated. LPA significantly increased rates cleavage blastocyst formation compared those seen un-treatment (control) group. The embryonic development-related genes (IGF2R, PCNA CDH1) higher (P < 0.05) treatment group than control numbers total, inner mass EdU (5-ethynyl-2’-deoxyuridine)-positive cells SCNT blastocysts TUNEL assay showed that reduced apoptosis rate embryos; this confirmed by decreases pro-apoptotic genes, BAX CASP3, an increase level anti-apoptotic gene, BCL2L1. In addition, Oct4 at gene protein levels. Together, our data suggest improves quality reducing enhancing pluripotency.